University of Central Florida (UCF) MCB3020C General Microbiology Lab Midterm Practice Exam

A complete yellow slant and butt in Kligler's Iron Agar (KIA) indicates that both lactose and glucose have been fermented. In a KIA test, the medium contains both glucose and lactose, and the yellow color signifies acid production as a result of fermentation.

When both sugars are utilized, the entire medium turns yellow due to the production of acid affecting both the slant (aerobic environment) and the butt (anaerobic environment). This provides a clear indication that both lactose and glucose are being fermented, which is consistent with the formation of acid throughout the medium. The presence of gas can also be indicated if there are cracks or bubbles in the agar, further confirming fermentation activity.

This distinctive result helps in differentiating between different types of bacteria based on their fermentation capabilities, making it an essential component in microbiological diagnostics. The other responses allude to scenarios where only one sugar is fermented or none, but those would not produce the full yellow color observed in both the slant and butt.

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