In Gram staining, what color do Gram-positive bacteria appear after the procedure?

In Gram staining, Gram-positive bacteria appear purple after the procedure. This result is due to the thick peptidoglycan layer in their cell walls, which retains the crystal violet dye used during the staining process. After the initial staining with crystal violet and a subsequent rinse, Gram-positive cells do not lose the dye during the alcohol decolorization step, leading to their characteristic purple coloration. This distinguishes them from Gram-negative bacteria, which have a thinner peptidoglycan layer and do not retain the crystal violet, resulting in a different color after the staining process.