Which test is used to detect the presence of cytochrome C oxidase?

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Prepare for the UCF General Microbiology Lab Midterm Exam. Study using flashcards and multiple choice questions, each question has hints and explanations. Get ready for your exam!

The test used to detect the presence of cytochrome C oxidase is the oxidase test. This test is based on the ability of certain bacteria to utilize cytochrome C as part of their electron transport chain in respiration. When the bacteria are incubated with a reagent that serves as an electron donor in the oxidase test, a color change indicates the presence of cytochrome C oxidase. This is typically visualized as a color change to purple within a few seconds, confirming the activity of the enzyme.

The other tests mentioned assess different factors or metabolic pathways in bacteria: the catalase test determines the presence of catalase, which breaks down hydrogen peroxide; the nitrate reduction test evaluates the ability of bacteria to reduce nitrate; and the viable plate count is a method for determining the number of live cells in a sample based on colony formation. Each of these tests serves a unique purpose that does not relate directly to cytochrome C oxidase detection.